C protein (beta gamma) subunit-mediated presynaptic inhibition: regulation of exocytotic fusion downstream of Ca2+ entry.

The nervous system can modulate neurotransmitter release by neurotransmitter activation of heterotrimeric GTPDbinding protein (G protein)Dcoupled receptors. We found that microinjection of G protein bg subunits (Gbg) mimics serotoninOs inhibitory effect on neurotransmission. Release of free Gbg was...

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Main Authors: Blackmer, Trillium., Larsen, Eric., Takahashi, Michiko., Martin, Thomas., Alford, Simon., Hamm, Heidi.
Format: Villanova Faculty Authorship
Language:English
Published: 2001
Online Access:http://ezproxy.villanova.edu/login?url=https://digital.library.villanova.edu/Item/vudl:178006
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spelling C protein (beta gamma) subunit-mediated presynaptic inhibition: regulation of exocytotic fusion downstream of Ca2+ entry.
Blackmer, Trillium.
Larsen, Eric.
Takahashi, Michiko.
Martin, Thomas.
Alford, Simon.
Hamm, Heidi.
The nervous system can modulate neurotransmitter release by neurotransmitter activation of heterotrimeric GTPDbinding protein (G protein)Dcoupled receptors. We found that microinjection of G protein bg subunits (Gbg) mimics serotoninOs inhibitory effect on neurotransmission. Release of free Gbg was critical for this effect because a Gbg scavenger blocked serotoninOs effect. Gbg had no effect on fast, action potentialDevoked intracellular Ca21 release that triggered neurotransmission. Inhibition of neurotransmitter release by serotonin was still seen after blockade of all classical Gbg effector pathways. Thus, Gbg blocked neurotransmitter release downstream of Ca21 entry and may directly target the exocytotic fusion machinery at the presynaptic terminal.
2001
Villanova Faculty Authorship
vudl:178006
Science 292, Apr 13, 2001, 293-297.
en
dc.title_txt_mv C protein (beta gamma) subunit-mediated presynaptic inhibition: regulation of exocytotic fusion downstream of Ca2+ entry.
dc.creator_txt_mv Blackmer, Trillium.
Larsen, Eric.
Takahashi, Michiko.
Martin, Thomas.
Alford, Simon.
Hamm, Heidi.
dc.description_txt_mv The nervous system can modulate neurotransmitter release by neurotransmitter activation of heterotrimeric GTPDbinding protein (G protein)Dcoupled receptors. We found that microinjection of G protein bg subunits (Gbg) mimics serotoninOs inhibitory effect on neurotransmission. Release of free Gbg was critical for this effect because a Gbg scavenger blocked serotoninOs effect. Gbg had no effect on fast, action potentialDevoked intracellular Ca21 release that triggered neurotransmission. Inhibition of neurotransmitter release by serotonin was still seen after blockade of all classical Gbg effector pathways. Thus, Gbg blocked neurotransmitter release downstream of Ca21 entry and may directly target the exocytotic fusion machinery at the presynaptic terminal.
dc.date_txt_mv 2001
dc.format_txt_mv Villanova Faculty Authorship
dc.identifier_txt_mv vudl:178006
dc.source_txt_mv Science 292, Apr 13, 2001, 293-297.
dc.language_txt_mv en
author Blackmer, Trillium.
Larsen, Eric.
Takahashi, Michiko.
Martin, Thomas.
Alford, Simon.
Hamm, Heidi.
spellingShingle Blackmer, Trillium.
Larsen, Eric.
Takahashi, Michiko.
Martin, Thomas.
Alford, Simon.
Hamm, Heidi.
C protein (beta gamma) subunit-mediated presynaptic inhibition: regulation of exocytotic fusion downstream of Ca2+ entry.
author_facet Blackmer, Trillium.
Larsen, Eric.
Takahashi, Michiko.
Martin, Thomas.
Alford, Simon.
Hamm, Heidi.
dc_source_str_mv Science 292, Apr 13, 2001, 293-297.
format Villanova Faculty Authorship
author_sort Blackmer, Trillium.
dc_date_str 2001
dc_title_str C protein (beta gamma) subunit-mediated presynaptic inhibition: regulation of exocytotic fusion downstream of Ca2+ entry.
description The nervous system can modulate neurotransmitter release by neurotransmitter activation of heterotrimeric GTPDbinding protein (G protein)Dcoupled receptors. We found that microinjection of G protein bg subunits (Gbg) mimics serotoninOs inhibitory effect on neurotransmission. Release of free Gbg was critical for this effect because a Gbg scavenger blocked serotoninOs effect. Gbg had no effect on fast, action potentialDevoked intracellular Ca21 release that triggered neurotransmission. Inhibition of neurotransmitter release by serotonin was still seen after blockade of all classical Gbg effector pathways. Thus, Gbg blocked neurotransmitter release downstream of Ca21 entry and may directly target the exocytotic fusion machinery at the presynaptic terminal.
title C protein (beta gamma) subunit-mediated presynaptic inhibition: regulation of exocytotic fusion downstream of Ca2+ entry.
title_full C protein (beta gamma) subunit-mediated presynaptic inhibition: regulation of exocytotic fusion downstream of Ca2+ entry.
title_fullStr C protein (beta gamma) subunit-mediated presynaptic inhibition: regulation of exocytotic fusion downstream of Ca2+ entry.
title_full_unstemmed C protein (beta gamma) subunit-mediated presynaptic inhibition: regulation of exocytotic fusion downstream of Ca2+ entry.
title_short C protein (beta gamma) subunit-mediated presynaptic inhibition: regulation of exocytotic fusion downstream of Ca2+ entry.
title_sort c protein (beta gamma) subunit-mediated presynaptic inhibition: regulation of exocytotic fusion downstream of ca2+ entry.
publishDate 2001
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language English
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