Toxic effects of thiol-reactive compounds on anaerobic biomass.

Toxic effects of thiol-reactive compounds on anaerobic biomass.

The objective of this study was to characterize the toxic effects of three well known thiol-reactive electrophilic compounds, Nethylmaleimide (NEM), pentachlorophenol (PCP) and 1-chloro-2,4-dinitrobenzene (CDNB) on anaerobic biotransformation process. The work was part of a larger investigation on p...

Full description

Bibliographic Details
Main Authors: Tepe, Nalan., Shimko, Leah., Duran, Metin.
Format: Villanova Faculty Authorship
Language:English
Published: 2005
Online Access:http://ezproxy.villanova.edu/login?url=https://digital.library.villanova.edu/Item/vudl:175960
id vudl:175960
record_format vudl
institution Villanova University
collection Digital Library
modeltype_str_mv vudl-system:CoreModel
vudl-system:ResourceCollection
vudl-system:CollectionModel
datastream_str_mv PROCESS-MD
PARENT-LIST-RAW
STRUCTMAP
LEGACY-METS
DC
PARENT-QUERY
PARENT-LIST
THUMBNAIL
MEMBER-LIST-RAW
AGENTS
RELS-EXT
MEMBER-QUERY
LICENSE
AUDIT
hierarchytype
hierarchy_all_parents_str_mv vudl:175911
vudl:172968
vudl:641262
vudl:3
vudl:1
sequence_vudl_175911_str 0000000017
hierarchy_top_id vudl:641262
hierarchy_top_title Villanova Faculty Publications
fedora_parent_id_str_mv vudl:175911
hierarchy_first_parent_id_str vudl:175960
hierarchy_parent_id vudl:175911
hierarchy_parent_title Duran Metin
hierarchy_sequence_sort_str 0000000017
hierarchy_sequence 0000000017
spelling Toxic effects of thiol-reactive compounds on anaerobic biomass.
Tepe, Nalan.
Shimko, Leah.
Duran, Metin.
The objective of this study was to characterize the toxic effects of three well known thiol-reactive electrophilic compounds, Nethylmaleimide (NEM), pentachlorophenol (PCP) and 1-chloro-2,4-dinitrobenzene (CDNB) on anaerobic biotransformation process. The work was part of a larger investigation on potassium efflux as a possible response mechanism of anaerobic microorganisms to the presence of thiol-reactive organic compounds and the interference of such compounds on the reductive dehalogenation process. Using anaerobic toxicity assay (ATA) and granular anaerobic biomass from a full-scale upflow anaerobic sludge blanket (UASB) reactor, inhibitory concentrations of these compounds that reduced the microbial activity of granular biomass to 50% of a control (IC50) were determined to be 592, 0.97, and 450 mg/l for NEM, PCP, and CDNB, respectively. Toxicity of NEM was also tested on anaerobic biomass from a municipal wastewater treatment plant digester and slightly lower IC50 of 532 mg/l was obtained. The results presented here indicate that anaerobic biomass can acclimate to the three thiol-reactive compounds studied and recover from inhibition as long as the toxicant concentration is below a threshold level. That threshold concentration was found to be 500 mg/l for NEM on biomass from the municipal digester, 1 mg/l for PCP, and 500 mg/l for CDNB, both on granular biomass. Granular anaerobic biomass showed recovery even at NEM concentrations of 1000 mg/l.
2005
Villanova Faculty Authorship
vudl:175960
Bioresource Technology 97, 2006, 592-598.
en
dc.title_txt_mv Toxic effects of thiol-reactive compounds on anaerobic biomass.
dc.creator_txt_mv Tepe, Nalan.
Shimko, Leah.
Duran, Metin.
dc.description_txt_mv The objective of this study was to characterize the toxic effects of three well known thiol-reactive electrophilic compounds, Nethylmaleimide (NEM), pentachlorophenol (PCP) and 1-chloro-2,4-dinitrobenzene (CDNB) on anaerobic biotransformation process. The work was part of a larger investigation on potassium efflux as a possible response mechanism of anaerobic microorganisms to the presence of thiol-reactive organic compounds and the interference of such compounds on the reductive dehalogenation process. Using anaerobic toxicity assay (ATA) and granular anaerobic biomass from a full-scale upflow anaerobic sludge blanket (UASB) reactor, inhibitory concentrations of these compounds that reduced the microbial activity of granular biomass to 50% of a control (IC50) were determined to be 592, 0.97, and 450 mg/l for NEM, PCP, and CDNB, respectively. Toxicity of NEM was also tested on anaerobic biomass from a municipal wastewater treatment plant digester and slightly lower IC50 of 532 mg/l was obtained. The results presented here indicate that anaerobic biomass can acclimate to the three thiol-reactive compounds studied and recover from inhibition as long as the toxicant concentration is below a threshold level. That threshold concentration was found to be 500 mg/l for NEM on biomass from the municipal digester, 1 mg/l for PCP, and 500 mg/l for CDNB, both on granular biomass. Granular anaerobic biomass showed recovery even at NEM concentrations of 1000 mg/l.
dc.date_txt_mv 2005
dc.format_txt_mv Villanova Faculty Authorship
dc.identifier_txt_mv vudl:175960
dc.source_txt_mv Bioresource Technology 97, 2006, 592-598.
dc.language_txt_mv en
author Tepe, Nalan.
Shimko, Leah.
Duran, Metin.
spellingShingle Tepe, Nalan.
Shimko, Leah.
Duran, Metin.
Toxic effects of thiol-reactive compounds on anaerobic biomass.
author_facet Tepe, Nalan.
Shimko, Leah.
Duran, Metin.
dc_source_str_mv Bioresource Technology 97, 2006, 592-598.
format Villanova Faculty Authorship
author_sort Tepe, Nalan.
dc_date_str 2005
dc_title_str Toxic effects of thiol-reactive compounds on anaerobic biomass.
description The objective of this study was to characterize the toxic effects of three well known thiol-reactive electrophilic compounds, Nethylmaleimide (NEM), pentachlorophenol (PCP) and 1-chloro-2,4-dinitrobenzene (CDNB) on anaerobic biotransformation process. The work was part of a larger investigation on potassium efflux as a possible response mechanism of anaerobic microorganisms to the presence of thiol-reactive organic compounds and the interference of such compounds on the reductive dehalogenation process. Using anaerobic toxicity assay (ATA) and granular anaerobic biomass from a full-scale upflow anaerobic sludge blanket (UASB) reactor, inhibitory concentrations of these compounds that reduced the microbial activity of granular biomass to 50% of a control (IC50) were determined to be 592, 0.97, and 450 mg/l for NEM, PCP, and CDNB, respectively. Toxicity of NEM was also tested on anaerobic biomass from a municipal wastewater treatment plant digester and slightly lower IC50 of 532 mg/l was obtained. The results presented here indicate that anaerobic biomass can acclimate to the three thiol-reactive compounds studied and recover from inhibition as long as the toxicant concentration is below a threshold level. That threshold concentration was found to be 500 mg/l for NEM on biomass from the municipal digester, 1 mg/l for PCP, and 500 mg/l for CDNB, both on granular biomass. Granular anaerobic biomass showed recovery even at NEM concentrations of 1000 mg/l.
title Toxic effects of thiol-reactive compounds on anaerobic biomass.
title_full Toxic effects of thiol-reactive compounds on anaerobic biomass.
title_fullStr Toxic effects of thiol-reactive compounds on anaerobic biomass.
title_full_unstemmed Toxic effects of thiol-reactive compounds on anaerobic biomass.
title_short Toxic effects of thiol-reactive compounds on anaerobic biomass.
title_sort toxic effects of thiol-reactive compounds on anaerobic biomass.
publishDate 2005
normalized_sort_date 2005-01-01T00:00:00Z
language English
collection_title_sort_str toxic effects of thiol-reactive compounds on anaerobic biomass.
relsext.hasModel_txt_mv http://hades.library.villanova.edu:8080/rest/vudl-system:CoreModel
http://hades.library.villanova.edu:8080/rest/vudl-system:ResourceCollection
http://hades.library.villanova.edu:8080/rest/vudl-system:CollectionModel
fgs.type_txt_mv http://www.w3.org/ns/ldp#Container
http://www.w3.org/ns/ldp#RDFSource
http://www.w3.org/ns/ldp#BasicContainer
http://fedora.info/definitions/v4/repository#Resource
http://www.w3.org/ns/ldp#Resource
http://fedora.info/definitions/v4/repository#Container
fgs.createdBy_txt_mv fedoraAdmin
fgs.createdDate_txt_mv 2013-01-22T05:10:12.750Z
relsext.itemID_txt_mv oai:digital.library.villanova.edu:vudl:175960
relsext.hasLegacyURL_txt_mv http://digital.library.villanova.edu/Villanova%20Digital%20Collection/Faculty%20Fulltext/Duran%20Metin/DuranMetin-756ef98d-aea1-4adc-adb2-d86b4928799f.xml
fgs.ownerId_txt_mv diglibEditor
relsext.sequence_txt_mv vudl:175911#17
fgs.lastModifiedBy_txt_mv fedoraAdmin
fgs.state_txt_mv Active
fgs.lastModifiedDate_txt_mv 2021-04-12T18:59:07.677Z
fgs.label_txt_mv Toxic effects of thiol-reactive compounds on anaerobic biomass.
relsext.isMemberOf_txt_mv http://hades.library.villanova.edu:8080/rest/vudl:175911
relsext.sortOn_txt_mv title
has_order_str no
agent.name_txt_mv Falvey Memorial Library, Villanova University
klk
license.mdRef_str http://digital.library.villanova.edu/copyright.html
license_str protected
has_thumbnail_str true
THUMBNAIL_contentDigest_digest_str 203c69e18f4f46c81e9892448d2c07cd
first_indexed 2014-01-11T23:13:21Z
last_indexed 2021-04-12T19:00:28Z
_version_ 1783101532371156992
subpages

Similar Items