Phenotypic characterization of Escherichia coli through whole-cell fatty acid profiling to investigate host specificity.

The objective of the study was to investigate whole-cell fatty acid methyl ester (FAME) profiles of 605 Escherichia coli isolates to determine their host specificity. The isolates were cultured from six possible sources of fecal pollution; 180 isolates from sewage, 85 from dairy cow, 98 from chicken, 76 from swine, 94 from deer, and 72 from waterfowl, mostly geese and ducks. The FAME profiles were presented as the relative masses of 12 FAMEs identified in the isolates and it was found that none of the six hosts carried a 'signature' FAME, a FAME that is uniquely associated with a particular host category. However, twosample t-test analyses indicated that the mean relative masses of seven FAMEs out of the 12 identified showed statistically significant differences (95% confidence interval) between isolates of human and non-human origins. In addition, a linear discriminant function based on mean relative mass variations in individual FAMEs classified the known-source isolates into their respective host categories with a 47.6% average rate of correct classification (ARCC) in a six-way discriminant analysis. The ARCC increased to 61.3% when the individual hosts were pooled into larger categories of human, livestock, and wildlife. The accuracy was 75.5% when isolates of human origin were discriminated against those of non-human origins. Random cluster formation analysis indicated that the library size was sufficient to prevent random grouping among the isolates.

Main Author: Haznedaroglu, Berat.
Other Authors: Yurtsever, Deniz., Lefkowitz, Jamie., Duran, Metin.
Language: English
Published: 2006
Online Access: http://ezproxy.villanova.edu/login?url=https://digital.library.villanova.edu/Item/vudl:175945
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dc_source_str_mv Water Research 41, 2007, 803-809.
author Haznedaroglu, Berat.
author_s Haznedaroglu, Berat.
spellingShingle Haznedaroglu, Berat.
Phenotypic characterization of Escherichia coli through whole-cell fatty acid profiling to investigate host specificity.
author-letter Haznedaroglu, Berat.
author_sort_str Haznedaroglu, Berat.
author2 Yurtsever, Deniz.
Lefkowitz, Jamie.
Duran, Metin.
author2Str Yurtsever, Deniz.
Lefkowitz, Jamie.
Duran, Metin.
dc_title_str Phenotypic characterization of Escherichia coli through whole-cell fatty acid profiling to investigate host specificity.
title Phenotypic characterization of Escherichia coli through whole-cell fatty acid profiling to investigate host specificity.
title_short Phenotypic characterization of Escherichia coli through whole-cell fatty acid profiling to investigate host specificity.
title_full Phenotypic characterization of Escherichia coli through whole-cell fatty acid profiling to investigate host specificity.
title_fullStr Phenotypic characterization of Escherichia coli through whole-cell fatty acid profiling to investigate host specificity.
title_full_unstemmed Phenotypic characterization of Escherichia coli through whole-cell fatty acid profiling to investigate host specificity.
collection_title_sort_str phenotypic characterization of escherichia coli through whole-cell fatty acid profiling to investigate host specificity.
title_sort phenotypic characterization of escherichia coli through whole-cell fatty acid profiling to investigate host specificity.
description The objective of the study was to investigate whole-cell fatty acid methyl ester (FAME) profiles of 605 Escherichia coli isolates to determine their host specificity. The isolates were cultured from six possible sources of fecal pollution; 180 isolates from sewage, 85 from dairy cow, 98 from chicken, 76 from swine, 94 from deer, and 72 from waterfowl, mostly geese and ducks. The FAME profiles were presented as the relative masses of 12 FAMEs identified in the isolates and it was found that none of the six hosts carried a 'signature' FAME, a FAME that is uniquely associated with a particular host category. However, twosample t-test analyses indicated that the mean relative masses of seven FAMEs out of the 12 identified showed statistically significant differences (95% confidence interval) between isolates of human and non-human origins. In addition, a linear discriminant function based on mean relative mass variations in individual FAMEs classified the known-source isolates into their respective host categories with a 47.6% average rate of correct classification (ARCC) in a six-way discriminant analysis. The ARCC increased to 61.3% when the individual hosts were pooled into larger categories of human, livestock, and wildlife. The accuracy was 75.5% when isolates of human origin were discriminated against those of non-human origins. Random cluster formation analysis indicated that the library size was sufficient to prevent random grouping among the isolates.
publishDate 2006
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fgs.label Phenotypic characterization of Escherichia coli through whole-cell fatty acid profiling to investigate host specificity.
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dc.title Phenotypic characterization of Escherichia coli through whole-cell fatty acid profiling to investigate host specificity.
dc.creator Haznedaroglu, Berat.
Yurtsever, Deniz.
Lefkowitz, Jamie.
Duran, Metin.
dc.description The objective of the study was to investigate whole-cell fatty acid methyl ester (FAME) profiles of 605 Escherichia coli isolates to determine their host specificity. The isolates were cultured from six possible sources of fecal pollution; 180 isolates from sewage, 85 from dairy cow, 98 from chicken, 76 from swine, 94 from deer, and 72 from waterfowl, mostly geese and ducks. The FAME profiles were presented as the relative masses of 12 FAMEs identified in the isolates and it was found that none of the six hosts carried a 'signature' FAME, a FAME that is uniquely associated with a particular host category. However, twosample t-test analyses indicated that the mean relative masses of seven FAMEs out of the 12 identified showed statistically significant differences (95% confidence interval) between isolates of human and non-human origins. In addition, a linear discriminant function based on mean relative mass variations in individual FAMEs classified the known-source isolates into their respective host categories with a 47.6% average rate of correct classification (ARCC) in a six-way discriminant analysis. The ARCC increased to 61.3% when the individual hosts were pooled into larger categories of human, livestock, and wildlife. The accuracy was 75.5% when isolates of human origin were discriminated against those of non-human origins. Random cluster formation analysis indicated that the library size was sufficient to prevent random grouping among the isolates.
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