Oleosin genes in maize kernels having diverse oil contents are constitutively expressed independent of oil contents.
In seeds, the subcellular storage oil bodies have a matrix of oils (triacylglycerols) surrounded by a layer of phospholipids embedded with abundant structural proteins called oleosins. We used two maize (Zea mays L.) strains having diverse kernel (seed) oil contents to study the effects of varying t...
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Language: | English |
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1995
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Oleosin genes in maize kernels having diverse oil contents are constitutively expressed independent of oil contents. Ting, Julie. Lee, Keunmyoung. Ratnayake, Chandra. Platt, Kathryn. Balsamo, Ronald. Huang, Anthony. In seeds, the subcellular storage oil bodies have a matrix of oils (triacylglycerols) surrounded by a layer of phospholipids embedded with abundant structural proteins called oleosins. We used two maize (Zea mays L.) strains having diverse kernel (seed) oil contents to study the effects of varying the oil and oleosin contents on the structure of the oil bodies. Illinois High Oils (IHO, 15% w/w oils) and Illinois Low Oils (ILO, 0.5%) maize kernels were the products of breeding for diverse oil contents for about 100 generations. In both maize strains, although the genes for oil synthesis had apparently been modified drastically, the genes encoding oleosins appeared to be unaltered, as revealed by Southern blot analyses of the three oleosin genes and sodium dodecyl sulfate-polyacrylamide gel electrophoresis with immunoblotting of the oleosins. In addition, both strains contained the same three oleosin isoforms of a defined proportion, and both accumulated oils and oleosins coordinately. Oleosins in both strains were restricted to the oil bodies, as shown by analyses of the various subcellular fractions separated by sucrosedensity- gradient centrifugation. Electron microscopy of the embryos and the isolated organdies revealed that the oil bodies in IHO were larger and had a spherical shape, whereas those in ILO were smaller and had irregular shapes. We conclude that in seeds, oleosin genes are expressed independent of the oil contents, and the size and shape of the oil bodies are dictated by the ratio of oils to oleosins synthesized during seed maturation. The extensive breeding for diverse oil contents has not altered the apparent mechanism of oil-body synthesis and the occurrence of hetero-dimer or -multimer of oleosin isoforms on the oil bodies. 1995 Villanova Faculty Authorship vudl:173970 Planta 199(1), 1996, 158-165. en |
dc.title_txt_mv |
Oleosin genes in maize kernels having diverse oil contents are constitutively expressed independent of oil contents. |
dc.creator_txt_mv |
Ting, Julie. Lee, Keunmyoung. Ratnayake, Chandra. Platt, Kathryn. Balsamo, Ronald. Huang, Anthony. |
dc.description_txt_mv |
In seeds, the subcellular storage oil bodies have
a matrix of oils (triacylglycerols) surrounded by a layer of
phospholipids embedded with abundant structural proteins
called oleosins. We used two maize (Zea mays L.)
strains having diverse kernel (seed) oil contents to study
the effects of varying the oil and oleosin contents on the
structure of the oil bodies. Illinois High Oils (IHO, 15%
w/w oils) and Illinois Low Oils (ILO, 0.5%) maize kernels
were the products of breeding for diverse oil contents for
about 100 generations. In both maize strains, although the
genes for oil synthesis had apparently been modified drastically,
the genes encoding oleosins appeared to be unaltered,
as revealed by Southern blot analyses of the three
oleosin genes and sodium dodecyl sulfate-polyacrylamide
gel electrophoresis with immunoblotting of the oleosins.
In addition, both strains contained the same three oleosin
isoforms of a defined proportion, and both accumulated
oils and oleosins coordinately. Oleosins in both strains
were restricted to the oil bodies, as shown by analyses of
the various subcellular fractions separated by sucrosedensity-
gradient centrifugation. Electron microscopy of
the embryos and the isolated organdies revealed that the
oil bodies in IHO were larger and had a spherical shape,
whereas those in ILO were smaller and had irregular
shapes. We conclude that in seeds, oleosin genes are expressed
independent of the oil contents, and the size and
shape of the oil bodies are dictated by the ratio of oils to
oleosins synthesized during seed maturation. The extensive
breeding for diverse oil contents has not altered the
apparent mechanism of oil-body synthesis and the occurrence
of hetero-dimer or -multimer of oleosin isoforms on
the oil bodies. |
dc.date_txt_mv |
1995 |
dc.format_txt_mv |
Villanova Faculty Authorship |
dc.identifier_txt_mv |
vudl:173970 |
dc.source_txt_mv |
Planta 199(1), 1996, 158-165. |
dc.language_txt_mv |
en |
author |
Ting, Julie. Lee, Keunmyoung. Ratnayake, Chandra. Platt, Kathryn. Balsamo, Ronald. Huang, Anthony. |
spellingShingle |
Ting, Julie. Lee, Keunmyoung. Ratnayake, Chandra. Platt, Kathryn. Balsamo, Ronald. Huang, Anthony. Oleosin genes in maize kernels having diverse oil contents are constitutively expressed independent of oil contents. |
author_facet |
Ting, Julie. Lee, Keunmyoung. Ratnayake, Chandra. Platt, Kathryn. Balsamo, Ronald. Huang, Anthony. |
dc_source_str_mv |
Planta 199(1), 1996, 158-165. |
format |
Villanova Faculty Authorship |
author_sort |
Ting, Julie. |
dc_date_str |
1995 |
dc_title_str |
Oleosin genes in maize kernels having diverse oil contents are constitutively expressed independent of oil contents. |
description |
In seeds, the subcellular storage oil bodies have
a matrix of oils (triacylglycerols) surrounded by a layer of
phospholipids embedded with abundant structural proteins
called oleosins. We used two maize (Zea mays L.)
strains having diverse kernel (seed) oil contents to study
the effects of varying the oil and oleosin contents on the
structure of the oil bodies. Illinois High Oils (IHO, 15%
w/w oils) and Illinois Low Oils (ILO, 0.5%) maize kernels
were the products of breeding for diverse oil contents for
about 100 generations. In both maize strains, although the
genes for oil synthesis had apparently been modified drastically,
the genes encoding oleosins appeared to be unaltered,
as revealed by Southern blot analyses of the three
oleosin genes and sodium dodecyl sulfate-polyacrylamide
gel electrophoresis with immunoblotting of the oleosins.
In addition, both strains contained the same three oleosin
isoforms of a defined proportion, and both accumulated
oils and oleosins coordinately. Oleosins in both strains
were restricted to the oil bodies, as shown by analyses of
the various subcellular fractions separated by sucrosedensity-
gradient centrifugation. Electron microscopy of
the embryos and the isolated organdies revealed that the
oil bodies in IHO were larger and had a spherical shape,
whereas those in ILO were smaller and had irregular
shapes. We conclude that in seeds, oleosin genes are expressed
independent of the oil contents, and the size and
shape of the oil bodies are dictated by the ratio of oils to
oleosins synthesized during seed maturation. The extensive
breeding for diverse oil contents has not altered the
apparent mechanism of oil-body synthesis and the occurrence
of hetero-dimer or -multimer of oleosin isoforms on
the oil bodies. |
title |
Oleosin genes in maize kernels having diverse oil contents are constitutively expressed independent of oil contents. |
title_full |
Oleosin genes in maize kernels having diverse oil contents are constitutively expressed independent of oil contents. |
title_fullStr |
Oleosin genes in maize kernels having diverse oil contents are constitutively expressed independent of oil contents. |
title_full_unstemmed |
Oleosin genes in maize kernels having diverse oil contents are constitutively expressed independent of oil contents. |
title_short |
Oleosin genes in maize kernels having diverse oil contents are constitutively expressed independent of oil contents. |
title_sort |
oleosin genes in maize kernels having diverse oil contents are constitutively expressed independent of oil contents. |
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1995 |
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