Oleosin genes in maize kernels having diverse oil contents are constitutively expressed independent of oil contents.

In seeds, the subcellular storage oil bodies have a matrix of oils (triacylglycerols) surrounded by a layer of phospholipids embedded with abundant structural proteins called oleosins. We used two maize (Zea mays L.) strains having diverse kernel (seed) oil contents to study the effects of varying the oil and oleosin contents on the structure of the oil bodies. Illinois High Oils (IHO, 15% w/w oils) and Illinois Low Oils (ILO, 0.5%) maize kernels were the products of breeding for diverse oil contents for about 100 generations. In both maize strains, although the genes for oil synthesis had apparently been modified drastically, the genes encoding oleosins appeared to be unaltered, as revealed by Southern blot analyses of the three oleosin genes and sodium dodecyl sulfate-polyacrylamide gel electrophoresis with immunoblotting of the oleosins. In addition, both strains contained the same three oleosin isoforms of a defined proportion, and both accumulated oils and oleosins coordinately. Oleosins in both strains were restricted to the oil bodies, as shown by analyses of the various subcellular fractions separated by sucrosedensity- gradient centrifugation. Electron microscopy of the embryos and the isolated organdies revealed that the oil bodies in IHO were larger and had a spherical shape, whereas those in ILO were smaller and had irregular shapes. We conclude that in seeds, oleosin genes are expressed independent of the oil contents, and the size and shape of the oil bodies are dictated by the ratio of oils to oleosins synthesized during seed maturation. The extensive breeding for diverse oil contents has not altered the apparent mechanism of oil-body synthesis and the occurrence of hetero-dimer or -multimer of oleosin isoforms on the oil bodies.

Main Author: Ting, Julie.
Other Authors: Lee, Keunmyoung., Ratnayake, Chandra., Platt, Kathryn., Balsamo, Ronald., Huang, Anthony.
Language: English
Published: 1995
Online Access: http://ezproxy.villanova.edu/login?url=https://digital.library.villanova.edu/Item/vudl:173970
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dc_source_str_mv Planta 199(1), 1996, 158-165.
author Ting, Julie.
author_facet_str_mv Ting, Julie.
Lee, Keunmyoung.
Ratnayake, Chandra.
Platt, Kathryn.
Balsamo, Ronald.
Huang, Anthony.
author_or_contributor_facet_str_mv Ting, Julie.
Lee, Keunmyoung.
Ratnayake, Chandra.
Platt, Kathryn.
Balsamo, Ronald.
Huang, Anthony.
author_s Ting, Julie.
spellingShingle Ting, Julie.
Oleosin genes in maize kernels having diverse oil contents are constitutively expressed independent of oil contents.
author-letter Ting, Julie.
author_sort_str Ting, Julie.
author2 Lee, Keunmyoung.
Ratnayake, Chandra.
Platt, Kathryn.
Balsamo, Ronald.
Huang, Anthony.
author2Str Lee, Keunmyoung.
Ratnayake, Chandra.
Platt, Kathryn.
Balsamo, Ronald.
Huang, Anthony.
dc_title_str Oleosin genes in maize kernels having diverse oil contents are constitutively expressed independent of oil contents.
title Oleosin genes in maize kernels having diverse oil contents are constitutively expressed independent of oil contents.
title_short Oleosin genes in maize kernels having diverse oil contents are constitutively expressed independent of oil contents.
title_full Oleosin genes in maize kernels having diverse oil contents are constitutively expressed independent of oil contents.
title_fullStr Oleosin genes in maize kernels having diverse oil contents are constitutively expressed independent of oil contents.
title_full_unstemmed Oleosin genes in maize kernels having diverse oil contents are constitutively expressed independent of oil contents.
collection_title_sort_str oleosin genes in maize kernels having diverse oil contents are constitutively expressed independent of oil contents.
title_sort oleosin genes in maize kernels having diverse oil contents are constitutively expressed independent of oil contents.
description In seeds, the subcellular storage oil bodies have a matrix of oils (triacylglycerols) surrounded by a layer of phospholipids embedded with abundant structural proteins called oleosins. We used two maize (Zea mays L.) strains having diverse kernel (seed) oil contents to study the effects of varying the oil and oleosin contents on the structure of the oil bodies. Illinois High Oils (IHO, 15% w/w oils) and Illinois Low Oils (ILO, 0.5%) maize kernels were the products of breeding for diverse oil contents for about 100 generations. In both maize strains, although the genes for oil synthesis had apparently been modified drastically, the genes encoding oleosins appeared to be unaltered, as revealed by Southern blot analyses of the three oleosin genes and sodium dodecyl sulfate-polyacrylamide gel electrophoresis with immunoblotting of the oleosins. In addition, both strains contained the same three oleosin isoforms of a defined proportion, and both accumulated oils and oleosins coordinately. Oleosins in both strains were restricted to the oil bodies, as shown by analyses of the various subcellular fractions separated by sucrosedensity- gradient centrifugation. Electron microscopy of the embryos and the isolated organdies revealed that the oil bodies in IHO were larger and had a spherical shape, whereas those in ILO were smaller and had irregular shapes. We conclude that in seeds, oleosin genes are expressed independent of the oil contents, and the size and shape of the oil bodies are dictated by the ratio of oils to oleosins synthesized during seed maturation. The extensive breeding for diverse oil contents has not altered the apparent mechanism of oil-body synthesis and the occurrence of hetero-dimer or -multimer of oleosin isoforms on the oil bodies.
publishDate 1995
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fgs.label Oleosin genes in maize kernels having diverse oil contents are constitutively expressed independent of oil contents.
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dc.title Oleosin genes in maize kernels having diverse oil contents are constitutively expressed independent of oil contents.
dc.creator Ting, Julie.
Lee, Keunmyoung.
Ratnayake, Chandra.
Platt, Kathryn.
Balsamo, Ronald.
Huang, Anthony.
dc.description In seeds, the subcellular storage oil bodies have a matrix of oils (triacylglycerols) surrounded by a layer of phospholipids embedded with abundant structural proteins called oleosins. We used two maize (Zea mays L.) strains having diverse kernel (seed) oil contents to study the effects of varying the oil and oleosin contents on the structure of the oil bodies. Illinois High Oils (IHO, 15% w/w oils) and Illinois Low Oils (ILO, 0.5%) maize kernels were the products of breeding for diverse oil contents for about 100 generations. In both maize strains, although the genes for oil synthesis had apparently been modified drastically, the genes encoding oleosins appeared to be unaltered, as revealed by Southern blot analyses of the three oleosin genes and sodium dodecyl sulfate-polyacrylamide gel electrophoresis with immunoblotting of the oleosins. In addition, both strains contained the same three oleosin isoforms of a defined proportion, and both accumulated oils and oleosins coordinately. Oleosins in both strains were restricted to the oil bodies, as shown by analyses of the various subcellular fractions separated by sucrosedensity- gradient centrifugation. Electron microscopy of the embryos and the isolated organdies revealed that the oil bodies in IHO were larger and had a spherical shape, whereas those in ILO were smaller and had irregular shapes. We conclude that in seeds, oleosin genes are expressed independent of the oil contents, and the size and shape of the oil bodies are dictated by the ratio of oils to oleosins synthesized during seed maturation. The extensive breeding for diverse oil contents has not altered the apparent mechanism of oil-body synthesis and the occurrence of hetero-dimer or -multimer of oleosin isoforms on the oil bodies.
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