Identification, subcellular localization, and developmental studies of oleosins in the anther of Brassica napus.

mRNAs encoding putative oleosins have been detected in the tapetum of developing anthers in Brassica and Arabidopsis, but the authentic proteins have not been previously documented. Antibodies against a synthetic 15-residue polypeptide that represents a portion of the putative tapetum oleosins encoded by two cloned Brassica napus genes were raised. Using these antibodies for immunoblotting after SDS-PAGE of the sporophytic extracts of B. napus developing anthers, two oleosins of ∼ 48 and 45 kDa were detected. These two oleosins were judged to be the putative oleosins encoded by cloned Brassica genes because of their identical N-terminal sequences. The two oleosins were present in the anthers only during the developmental stage when the tapetum cells were packed with organelles. A fraction of low-density organelles was isolated from the developing anthers by flotation centrifugation. The fraction contained plastoglobule-filled plastids and lipid-containing particles. The structures of these two isolated organelles were similar to those in situ in the tapetum cells. Of subcellular fractions of the anther homogenate, the two oleosins were present exclusively in the low-density organelle fraction. They were absent in the surface fractions of the developing microspores and the mature pollen, although fragmented oleosin molecules were earlier reported to be present on the pollen. By immunocytochemistry, immunogold particles were found largely on the periphery of the plastoglobuli inside the plastids in the tapetum cells. The antibodies also detected oleosins on the surface of storage oil bodies inside the maturing microspores. Apparently, the gametophytic microspore oil-body oleosins share common epitopes at the generally non-conserved C-terminal domain with the sporophytic tapetum oleosins.

Main Author: Wang, Tzann-Wei.
Other Authors: Balsamo, Ronald., Ratnayake, Chandra., Platt, Kathryn., Ting, Julie., Huang, Anthony.
Format: Villanova Faculty Authorship
Language: English
Published: 1996
Online Access: http://ezproxy.villanova.edu/login?url=https://digital.library.villanova.edu/Item/vudl:173946
PID vudl:173946
id vudl:173946
modeltype_str_mv vudl-system:CoreModel
vudl-system:CollectionModel
vudl-system:ResourceCollection
datastream_str_mv DC
PARENT-QUERY
PARENT-LIST-RAW
PARENT-LIST
MEMBER-QUERY
MEMBER-LIST-RAW
LEGACY-METS
LICENSE
AGENTS
PROCESS-MD
THUMBNAIL
STRUCTMAP
RELS-EXT
hierarchytype
sequence_vudl_173930_str 0000000006
has_order_str no
hierarchy_top_id vudl:641262
hierarchy_top_title Villanova faculty author
hierarchy_parent_id vudl:173930
hierarchy_parent_title Balsamo Ronald
hierarchy_sequence 0000000006
hierarchy_first_parent_id_str vudl:173946
hierarchy_sequence_sort_str 0000000006
hierarchy_all_parents_str_mv vudl:641262
vudl:172968
vudl:173930
first_indexed 2014-01-11T22:53:12Z
last_indexed 2021-04-12T19:42:00Z
recordtype vudl
fullrecord <root> <url> http://digital.library.villanova.edu/files/vudl:173946/DC </url> <thumbnail> http://digital.library.villanova.edu/files/vudl:173946/THUMBNAIL </thumbnail> </root>
spelling
institution Villanova University
collection Digital Library
language English
dc_source_str_mv The Plant Journal 11(3), 1997, 475-487.
author Wang, Tzann-Wei.
author_facet_str_mv Wang, Tzann-Wei.
Balsamo, Ronald.
Ratnayake, Chandra.
Platt, Kathryn.
Ting, Julie.
Huang, Anthony.
author_or_contributor_facet_str_mv Wang, Tzann-Wei.
Balsamo, Ronald.
Ratnayake, Chandra.
Platt, Kathryn.
Ting, Julie.
Huang, Anthony.
author_s Wang, Tzann-Wei.
spellingShingle Wang, Tzann-Wei.
Identification, subcellular localization, and developmental studies of oleosins in the anther of Brassica napus.
author-letter Wang, Tzann-Wei.
author_sort_str Wang, Tzann-Wei.
author2 Balsamo, Ronald.
Ratnayake, Chandra.
Platt, Kathryn.
Ting, Julie.
Huang, Anthony.
author2Str Balsamo, Ronald.
Ratnayake, Chandra.
Platt, Kathryn.
Ting, Julie.
Huang, Anthony.
dc_title_str Identification, subcellular localization, and developmental studies of oleosins in the anther of Brassica napus.
title Identification, subcellular localization, and developmental studies of oleosins in the anther of Brassica napus.
title_short Identification, subcellular localization, and developmental studies of oleosins in the anther of Brassica napus.
title_full Identification, subcellular localization, and developmental studies of oleosins in the anther of Brassica napus.
title_fullStr Identification, subcellular localization, and developmental studies of oleosins in the anther of Brassica napus.
title_full_unstemmed Identification, subcellular localization, and developmental studies of oleosins in the anther of Brassica napus.
collection_title_sort_str identification, subcellular localization, and developmental studies of oleosins in the anther of brassica napus.
title_sort identification, subcellular localization, and developmental studies of oleosins in the anther of brassica napus.
format Villanova Faculty Authorship
description mRNAs encoding putative oleosins have been detected in the tapetum of developing anthers in Brassica and Arabidopsis, but the authentic proteins have not been previously documented. Antibodies against a synthetic 15-residue polypeptide that represents a portion of the putative tapetum oleosins encoded by two cloned Brassica napus genes were raised. Using these antibodies for immunoblotting after SDS-PAGE of the sporophytic extracts of B. napus developing anthers, two oleosins of ∼ 48 and 45 kDa were detected. These two oleosins were judged to be the putative oleosins encoded by cloned Brassica genes because of their identical N-terminal sequences. The two oleosins were present in the anthers only during the developmental stage when the tapetum cells were packed with organelles. A fraction of low-density organelles was isolated from the developing anthers by flotation centrifugation. The fraction contained plastoglobule-filled plastids and lipid-containing particles. The structures of these two isolated organelles were similar to those in situ in the tapetum cells. Of subcellular fractions of the anther homogenate, the two oleosins were present exclusively in the low-density organelle fraction. They were absent in the surface fractions of the developing microspores and the mature pollen, although fragmented oleosin molecules were earlier reported to be present on the pollen. By immunocytochemistry, immunogold particles were found largely on the periphery of the plastoglobuli inside the plastids in the tapetum cells. The antibodies also detected oleosins on the surface of storage oil bodies inside the maturing microspores. Apparently, the gametophytic microspore oil-body oleosins share common epitopes at the generally non-conserved C-terminal domain with the sporophytic tapetum oleosins.
publishDate 1996
normalized_sort_date 1996-01-01T00:00:00Z
dc_date_str 1996
license_str protected
REPOSITORYNAME FgsRepos
REPOSBASEURL http://hades.library.villanova.edu:8088/fedora
fgs.state Active
fgs.label Identification, subcellular localization, and developmental studies of oleosins in the anther of Brassica napus.
fgs.ownerId diglibEditor
fgs.createdDate 2013-01-22T03:19:15.427Z
fgs.lastModifiedDate 2021-04-12T19:10:59.966Z
dc.title Identification, subcellular localization, and developmental studies of oleosins in the anther of Brassica napus.
dc.creator Wang, Tzann-Wei.
Balsamo, Ronald.
Ratnayake, Chandra.
Platt, Kathryn.
Ting, Julie.
Huang, Anthony.
dc.description mRNAs encoding putative oleosins have been detected in the tapetum of developing anthers in Brassica and Arabidopsis, but the authentic proteins have not been previously documented. Antibodies against a synthetic 15-residue polypeptide that represents a portion of the putative tapetum oleosins encoded by two cloned Brassica napus genes were raised. Using these antibodies for immunoblotting after SDS-PAGE of the sporophytic extracts of B. napus developing anthers, two oleosins of ∼ 48 and 45 kDa were detected. These two oleosins were judged to be the putative oleosins encoded by cloned Brassica genes because of their identical N-terminal sequences. The two oleosins were present in the anthers only during the developmental stage when the tapetum cells were packed with organelles. A fraction of low-density organelles was isolated from the developing anthers by flotation centrifugation. The fraction contained plastoglobule-filled plastids and lipid-containing particles. The structures of these two isolated organelles were similar to those in situ in the tapetum cells. Of subcellular fractions of the anther homogenate, the two oleosins were present exclusively in the low-density organelle fraction. They were absent in the surface fractions of the developing microspores and the mature pollen, although fragmented oleosin molecules were earlier reported to be present on the pollen. By immunocytochemistry, immunogold particles were found largely on the periphery of the plastoglobuli inside the plastids in the tapetum cells. The antibodies also detected oleosins on the surface of storage oil bodies inside the maturing microspores. Apparently, the gametophytic microspore oil-body oleosins share common epitopes at the generally non-conserved C-terminal domain with the sporophytic tapetum oleosins.
dc.date 1996
dc.format Villanova Faculty Authorship
dc.identifier vudl:173946
dc.source The Plant Journal 11(3), 1997, 475-487.
dc.language en
license.mdRef http://digital.library.villanova.edu/copyright.html
agent.name Falvey Memorial Library, Villanova University
klk
has_thumbnail true
THUMBNAIL_contentDigest_type MD5
THUMBNAIL_contentDigest_digest 203c69e18f4f46c81e9892448d2c07cd
THUMBNAIL_contentLocation_type INTERNAL_ID
THUMBNAIL_contentLocation_ref http://hades-vm.library.villanova.edu:8088/fedora/get/vudl:173946/THUMBNAIL/2013-01-22T03:19:17.557Z
relsext.hasModel info:fedora/vudl-system:CoreModel
info:fedora/vudl-system:CollectionModel
info:fedora/vudl-system:ResourceCollection
relsext.itemID oai:digital.library.villanova.edu:vudl:173946
relsext.isMemberOf info:fedora/vudl:173930
relsext.hasLegacyURL http://digital.library.villanova.edu/Villanova%20Digital%20Collection/Faculty%20Fulltext/Balsamo%20Ronald/BalsamoRonald-e083afd1-6229-4342-a5d2-106b830cd46e.xml
relsext.sortOn title
relsext.sequence vudl:173930#6
_version_ 1696864949636169728
score 13.586091
subpages