A colorimetric assay for the determination of polyhexamethylene biguanide in pool and spa water using nickel-nioxime.
A novel nickel-nioxime analytical method to measure polyhexamethylene biguanide (PHMB) in swimming pools and spas was developed. This method utilizes nickel(II) chloride and 1,2-cyclohexanedionedioxime (nioxime) chemistry. In the method, nickel ions bind and neutralize PHMB in the solution. Excess, un-reacted nickel ions react with nioxime and the resulting colored solution is measured at 550 nm using a colorimetric assay. Currently, the colorimetric method to measure PHMB uses bromophenol blue (BPB). However, high levels of quaternary ammonium based algaecides and surfactant based products interfere with this colorimetric method. A time-consuming and expensive high-performance liquid chromatographic (HPLC) analysis can be used for samples with high levels of quaternary ammonium based algaecides or surfactants. The proposed nickel-nioxime detection method achieves comparable PHMB results to HPLC in about 5 min and is a very economical and simple method to perform.
| Main Author: | Rowhani, Touraj. |
|---|---|
| Other Authors: | Lagalante, Anthony. |
| Format: | |
| Language: | English |
| Published: |
2006
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| Online Access: |
http://ezproxy.villanova.edu/login?url=https://digital.library.villanova.edu/Item/vudl:177853 |