Analysis of mass transport during warming of cryopreserved cells.
As a first step towards developing mechanistic models of damage to cryopreserved cells during the warming process, an analysis of water transport during warming was undertaken. With the aim of identifying regimes in which conventional twocompartment, membrane-limited water transport models will be valid for predicting cell dehydration/rehydration during warming, the Peclet number was evaluated. Simulations showed that Pe » 1 during the early stages of warming, indicating that transport was rate-limited by diffusion to and from the membrane. However, by comparing the time-scale of transport to the time-scale of the warming process, it was possible to identify conditions in which the membrane-limited transport model would yield adequate predictions in diffusion-dominated regimes. Simulations suggested that a membrane-limited model would fail during slow warming of rapidly cooled cells, but that it would perform adequately if cooling was slow, or if warming was rapid.
|Main Author:||Kasharin, Alexander V.|
|Other Authors:||Karlsson, Jens O. M.|